Viral DNA and protein translocation across nuclear
envelopes of plant and animal cells
Using mammalian pemebilized cells and Yeast based assays that allow
studying in-vitro and in-vivo nuclear import, we are analyzing the mechanistic
features of various nuclear viral proteins. These include the HIV-1 Rev, Tat and
Integrase which are essential viral proteins implied in the early and late
stages of infection. Moreover the Integrase protein has been suggested to be
involved in translocation of the viral Pre-Integration Complex (PIC) into the
host nucleus. Also, we are interested in the Gemini-virus BV1 movement protein
as well as in the TYLCV-virus capsid both-which have unique nuclear import
features, and were shown to be able to translocate the viral DNA across plant
cells via the plasmodesmata.
Nuclear
import of the BV1-NLS and CP-NLS peptides in microinjected plant cells.
Mesophyll cellsofN
.benthamianaleaves
were microinjected with the following transport
substrates (a) Rho–BSA–CP-NLS, (b)
Rho–BSA–BV1-NLS.
From:Kass,
G at el. Journal
of General Virology,
2006,
In press.
Copyright ©,2006 , The Hebrew University of Jerusalem. All Rights
Reserved
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