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Viral DNA and protein translocation across nuclear envelopes of plant and animal cells

Using mammalian pemebilized cells and Yeast based assays that allow studying in-vitro and in-vivo nuclear import, we are analyzing the mechanistic features of various nuclear viral proteins. These include the HIV-1 Rev, Tat and Integrase which are essential viral proteins implied in the early and late stages of infection. Moreover the Integrase protein has been suggested to be involved in translocation of the viral Pre-Integration Complex (PIC) into the host nucleus. Also, we are interested in the Gemini-virus BV1 movement protein as well as in the TYLCV-virus capsid both-which have unique nuclear import features, and were shown to be able to translocate the viral DNA across plant cells via the plasmodesmata. 

  

Nuclear import of the BV1-NLS and CP-NLS peptides in microinjected plant cells.  

Nuclear import of the BV1-NLS and CP-NLS peptides in microinjected plant cells. Mesophyll cellsofN .benthamianaleaves were microinjected with the following transport substrates (a) Rho–BSA–CP-NLS, (b) Rho–BSA–BV1-NLS.

From:Kass, G at el. Journal of General Virology, 2006, In press.

 

 

 

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